小鼠TSPAN8蛋白-ACROBiosystems百普赛斯

Mouse TSPAN8 Protein, His Tag (MALS verified)

分子别名（Synonym）

CO-029,tetraspanin 8,TM4SF3,TM4SF3tspan-8,Transmembrane 4 superfamily member 3tetraspanin-8,TSPAN8,Tspan-8,Tumor-associated antigen CO-029

表达区间及表达系统（Source）

Mouse TSPAN8 Protein, His Tag (TS8-M5249) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Asn 203 (Accession # Q8R3G9-1).

Predicted N-terminus: HiS

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 12.8 kDa. The protein migrates as 15-19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Tspan8是tetraspanin家族33个哺乳动物成员中的1个，由跨膜蛋白组成，这些蛋白横向组织，与整合素等其他膜伴侣一起或与整合素一起形成“tetraspanin-web”。这些平台在细胞内发出信号以调节许多细胞过程：粘附、迁移、侵袭或存活Tspan8与许多类型的癌症有关。据报道，在胶质瘤和结直肠癌、食管癌、肝癌、胃癌和胰腺癌中存在过表达。Tspan8通过与膜伴侣如α6β4整合素蛋白激酶C（PKC）激活、E-钙粘蛋白、EpCAM、claudin-7和CD44的结合来控制细胞和细胞-基质相互作用，从而发挥促侵袭功能。此外，Tspan8可能是一个有前景的新治疗靶点，因为Tspan8特异性抗体已被证明可以降低细胞运动，阻断体内肿瘤血管生成。