小鼠Siglec-10蛋白-ACROBiosystems百普赛斯

Mouse Siglec-10 Protein, His Tag

分子别名（Synonym）

SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

表达区间及表达系统（Source）

Mouse Siglec-10, His Tag (SI0-M52H7) is expressed from human 293 cells (HEK293). It contains AA Met 18 - Lys 543 (Accession # Q80ZE3-1).

Predicted N-terminus: Met 18

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 60.7 kDa. The protein migrates as 40 kDa,50 kDa and 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Siglecs（唾液酸结合Ig样凝集素）是具有粘附分子样结构的Ig超家族的一个独特子集。我们在这里描述了siglec蛋白家族的一个新成员，它具有相似的结构，包括五个Ig样结构域、一个跨膜结构域和一个含有两个ITIM信号基序的细胞质尾部。Siglec-10是通过哮喘嗜酸性粒细胞EST库的数据库挖掘鉴定的。Siglec-10-VAP-1相互作用似乎介导淋巴细胞与内皮细胞的粘附，并有可能通过酶终产物改变炎症微环境。