小鼠Klotho beta/KLB蛋白-ACROBiosystems百普赛斯

Mouse Klotho beta / KLB Protein, His Tag

分子别名（Synonym）

betaKlotho,beta-klotho,BKL,KLB,klotho beta like,klotho beta-like protein

表达区间及表达系统（Source）

Mouse Klotho beta, His Tag (KLB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 995 (Accession # Q99N32-1).

Predicted N-terminus: Phe 53

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 110.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

KLB（Klotho Beta）是一种蛋白质编码基因。其相关途径包括RET信号传导和HIV生命周期。与该基因相关的GO注释包括水解酶活性、水解O-糖基化合物和成纤维细胞生长因子结合。KL是该基因的一个重要同源物。Klothoβ是多种代谢过程的调节因子，但其在癌症中的作用尚不清楚。我们发现，与成对的相邻非肿瘤肝组织相比，βKlotho在人肝细胞癌组织中的表达下调。与正常肝细胞相比，肝癌细胞也显示出βKlotho表达降低。将βKlotho重新引入肝癌细胞抑制了它们的增殖。