小鼠MMP9蛋白-ACROBiosystems百普赛斯

Mouse MMP-9 (20-471) Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

MMP9,CLG4B,GELB,MANDP2,Gelatinase B

表达区间及表达系统（Source）

Mouse MMP-9 (20-471), His Tag (MM9-M52H1) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 471 (Accession # P41245-1). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

Predicted N-terminus: Ala 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 25 mM Tris,150 mM NaCl,pH7.5,50% Glycerol with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

基质金属肽酶9（MMP-9）也称为92 kDa IV型胶原酶、92 kDa明胶酶或明胶酶B（GELB）、CLG4B，由中性粒细胞、巨噬细胞和许多转化细胞分泌，是结构域结构和活性调节方面最复杂的家族成员。从结构上讲，MMP9可分为五个不同的结构域：一个在激活时被切割的前结构域，一个由三个连续的纤维连接蛋白II型单元组成的凝胶结合结构域，含有锌结合位点的催化结构域，富含脯氨酸的连接区，以及羧基末端的血红素样结构域。这种酶降解各种底物，包括明胶、IV型和V型胶原蛋白以及弹性蛋白。MMP9参与多种自身免疫性疾病，如系统性红斑狼疮、类风湿性关节炎和多发性硬化症，被视为潜在的治疗靶点。