重组人CD79A & CD79B蛋白-ACROBiosystems百普赛斯

Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (MALS verified)

分子别名（Synonym）

CD79A&CD79B, B-cell antigen receptor complex-associated protein alpha chain, B-cell antigen receptor complex-associated protein beta chain

表达区间及表达系统（Source）

Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (CDB-H52W3) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Arg 143 (CD79A) & Ala 29 - Asp 159 (CD79B) (Accession # P11912-1 (CD79A) & P40259-1 (CD79B)).

Predicted N-terminus: Leu 33 (CD79A) & Ala 29 (CD79B)

蛋白结构（Molecular Characterization）

Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag is produced by co-expression of CD79A and CD79B, has a calculated MW of 43.8 kDa (CD79A) and 46.7 kDa (CD79B). Subunit CD79A carries a mouse IgG2a Fc fragment at the C-terminus, followed by a flag tag and subunit CD79B carries a mouse IgG2a Fc fragment at the C-terminus, followed by a polyhistidine tag. The protein migrates as 120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD79a和CD79b异二聚体是B细胞受体（BCR）复合物的重要信号成分，在B细胞发育和抗体产生中起着至关重要的作用。BCR复合物由与信号成分非共价结合的配体结合受体（膜免疫球蛋白；mIg）、CD79a（Igα）和CD79b（Igβ）的二硫键连接异二聚体组成。这两种CD79亚基都由一个Ig样结构域、一个跨膜（TM）区域和一个较长的细胞质尾部（CYT）组成，该尾部包含一个基于免疫受体酪氨酸的激活基序（ITAM）。