小鼠FcRn (FCGRT & B2M)蛋白-ACROBiosystems百普赛斯

Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified)

分子别名（Synonym）

FcRn,FCGRT & B2M

表达区间及表达系统（Source）

Mouse FCGRT&B2M Heterodimer Protein, His Tag (FCM-M52W8) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).

Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)

蛋白结构（Molecular Characterization）

Mouse FCGRT&B2M Heterodimer Protein, His Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 40-50 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

FCGRT和B2M异二聚体蛋白（FcRn复合物）由两个亚基组成：p51（相当于FCGRT）和p14（相当于β-2-微球蛋白），形成MHC I类异二聚物。IgG、受体、转运蛋白、α（FCGRT）的Fc片段与单体免疫球蛋白γ的Fc区结合，并介导从牛奶中摄取IgG。FCGRT可能在免疫球蛋白G从母体转移到胎儿中发挥作用。β2-微球蛋白（B2M）是I类主要组织相容性复合体（MHC）的一种成分，参与向免疫系统呈递肽抗原。