小鼠IL2RB&IL2RG蛋白-ACROBiosystems百普赛斯

Mouse IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

分子别名（Synonym）

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统（Source）

Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (ILG-M5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 240 (IL-2RB) & Trp 23 - Ala 263 (IL-2RG) (Accession # P16297-1 (IL-2RB) & P34902-1 (IL-2RG)).

蛋白结构（Molecular Characterization）

Mouse IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.4 kDa (IL-2RB) and 35.0 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The protein migrates as 50-55 kDa and 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-2受体亚基β和白细胞介蛋白-2受体亚单位γ都是白细胞介介素-2的受体。多种白细胞介素受体的共同亚基。白细胞介素刺激后与SHB相互作用。可能与IL15RA有关，IL15参与刺激中性粒细胞吞噬作用。这种β亚基参与受体介导的内吞作用，并转导IL2的促有丝分裂信号。IL2R以3种不同的形式存在：高亲和力二聚体、中等亲和力单体（β亚基）和低亲和力单体。高亲和力和中等亲和力形式也与γ亚基相关。