小鼠IL-21蛋白-ACROBiosystems百普赛斯

Mouse IL-21 Protein, His Tag

分子别名（Synonym）

IL21,Za11,Interleukin-21

表达区间及表达系统（Source）

Mouse IL-21 Protein, His Tag (IL1-M52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Ser 146 (Accession # Q9ES17).

Predicted N-terminus: Pro 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.3 kDa. The protein migrates as 20 kDa and 23-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-21（IL-21）是一种分泌蛋白，属于IL-15/IL-21家族。白细胞介素-21/IL-21属于细胞因子家族，与由私人受体（IL21R）和常见细胞因子受体γ链（γ（C））组成的复合受体结合。白细胞介素-21/IL-21影响多种细胞类型，包括CD8+记忆T细胞、NK细胞和CD4记忆T细胞亚群。IL-21R广泛分布于淋巴造血细胞上。IL-21是CD4+T细胞在抗原刺激下产生的多效性细胞因子。它的作用通常会增强免疫细胞的抗原特异性反应。IL-21促进CD8+T细胞和NK细胞的抗肿瘤活性。IL-21通过与特定的I型细胞因子受体IL-21R结合发挥其作用，IL-21R还含有在其他细胞因子受体中发现的γ链（γc），包括IL-2、IL-4、IL-7、IL-9和IL-15。IL-21/IL-21R相互作用触发一系列事件，包括酪氨酸激酶JAK1和JAK3的激活，随后是转录因子STAT1和STAT3的激活。