小鼠IGF-I R/CD221蛋白-ACROBiosystems百普赛斯

Mouse IGF-I R / CD221 Protein, His Tag

分子别名（Synonym）

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表达区间及表达系统（Source）

Mouse IGF-I R, His Tag (IGR-M5223) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1).

Predicted N-terminus: Glu 31 & Asp 742

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.7 kDa and 24.3 kDa respectively. The protein migrates as 45-55 kDa (partial β chain), 100-125 kDa (α chain) and 150 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胰岛素样生长因子1受体（IGF1）也被称为CD221、JTK13。并且是由IGF-1和相关生长因子IGF-2激活的跨膜受体。它属于酪氨酸激酶受体的大类。这种受体介导IGF-1的作用，IGF-1是一种分子结构与胰岛素相似的多肽蛋白激素。IGF1R由两个α亚基和两个β亚基组成，α和β亚基都是由单个mRNA前体合成的。然后，前体被糖基化、蛋白水解切割，并通过半胱氨酸键交联，形成功能性的跨膜αβ链。α链位于细胞外，而β亚基横跨膜，负责配体刺激下的细胞内信号转导。IGF1R具有ATP的结合位点，ATP用于为自磷酸化提供磷酸盐。IGF1R和胰岛素受体之间有60%的同源性。作为配体结合的反应，α链诱导β链的酪氨酸自磷酸化。这一事件触发了一系列细胞内信号传导，虽然有点细胞类型特异性，但通常会促进细胞存活和细胞增殖。