小鼠H-2Kb&B2M&OVA四聚体蛋白-ACROBiosystems百普赛斯

PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein

表达区间及表达系统（Source）

PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein (H2A-MP2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) & SIINFEKL peptide (Accession # P01901 (H-2Kb) & P01887 (B2M) & SIINFEKL).

Predicted N-terminus: Gly 22 & Ile 21

蛋白结构（Molecular Characterization）

PE-Labeled Mouse H-2Kb&B2M&OVA (SIINFEKL) Tetramer Protein is assembled by biotinylated monomer (H2A-M82E6) and PE-labeled streptavidin.

Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein is produced by co-expression of H-2Kb and B2M loaded with OVA peptide. Biotinylated Mouse H-2Kb&B2M&OVA (SIINFEKL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

卵清蛋白（OVA）可以基于特征明确的肽表位诱导体液和细胞免疫反应。OVA257-264八肽是首批被鉴定的OVA表位之一，其氨基酸序列为SIINFEKL，可被细胞毒性T淋巴细胞识别。如果卵清蛋白（Ova）通过皮诺体的渗透裂解从皮诺体释放到细胞质中，则会被呈递给T细胞。佐剂SIINFEKL肽免疫诱导小鼠长期CD8+T细胞免疫。