小鼠IL-33蛋白-ACROBiosystems百普赛斯

Mouse IL-33 Protein, His Tag

分子别名（Synonym）

IL33,DV27,C9ORF26,IL1F11,NFHEV,DKFZp586H0523,DVS27,NFEHEV,RP11-575C20.2

表达区间及表达系统（Source）

Mouse IL-33, His Tag (IL3-M5245) is expressed from human 293 cells (HEK293). It contains AA Ser 109 - Ile 266 (Accession # Q8BVZ5-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.5 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素33（IL33）被称为C9orf26、DKFZp586H0523、DVS27、NF-HEV、NFEHEV、RP11-575C20.2，是一种属于IL-1超家族的细胞因子。IL-33诱导辅助性T细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞产生2型细胞因子。IL-33通过与受体ST2（又名IL1RL1）和IL-1受体辅助蛋白（IL1RAP）相互作用，激活NF-κB和MAP激酶信号通路中的细胞内分子，驱动极化Th2细胞产生2型细胞因子（如IL-5和IL-13），从而介导其生物学效应。在体内，IL-33诱导IL-4、IL-5和IL-13的表达，并导致粘膜器官的严重病理变化。