小鼠CD3E & CD3D蛋白-ACROBiosystems百普赛斯

Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

分子别名（Synonym）

CD3E & CD3D,CD3 delta & CD3 epsilon

表达区间及表达系统（Source）

Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

蛋白结构（Molecular Characterization）

Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 18-19 kDa and 25-32 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

T细胞表面糖蛋白CD3δ和CD3ε链，也称为CD3D和CD3E或CD3D和CD34E，是单程I型膜蛋白。CD3D与CD3-ε（CD3E）、CD3-γ和CD3ζ以及T细胞受体α/β和γ/δ异二聚体一起形成T细胞受体-CD3复合物。T细胞受体-CD3复合物在将抗原识别与几种细胞内信号转导通路偶联方面起着重要作用。