康普瑞汀|T6212|TargetMol

Combretastatin A4
117048-59-6

￥140 20mg 起订

上海更新日期：2024-09-23

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 康普瑞汀

英文名称：: Combretastatin A4

CAS号：: 117048-59-6

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.81%

产品类别：: 抑制剂

货号：: T6212

Product Introduction

Bioactivity

名称	Combretastatin A4
描述	Combretastatin A4 (CA4) is a microtubule-targeting agent that binds β-tubulin (Kd: 0.4 μM).
细胞实验	MDA-MB-231, A549, and HeLa cells are grown in DMEM medium (115 units/mL of penicillin G, 115 μg/mL of streptomycin, and 10% fetal bovine serum). Cells are seeded in 96-well plates (5000 cells/well) containing 50 μL of growth medium for 24 h. After medium removal, 100 μL of fresh medium containing individual analogue compounds at di?erent concentrations is added to each well and incubated at 37 ℃ for 72 h. After 24 h of culture, the cells are supplemented with 50 μL of analogue compounds dissolved in DMSO (less than 0.25% in each preparation). After 72 h of incubation, 20 μL of resazurin is added for 2 h before recording ?uorescence at 560 nm (excitation) and 590 nm (emission) using a Victor microtiter plate ?uorimeter. The IC50 is de?ned as the compound concentration required to inhibit cell proliferation by 50% in comparison with cells treated with the maximum amount of DMSO (0.25%) and considered as 100% viability.(Only for Reference)
激酶实验	Competitive binding assay using LC-MS/MS: Colchicine (1.2 μ M) is incubated with tubulin (1.3 mg/mL) in the incubation bu?er (80 mM PIPES, 2.0 mM MgCl2, 0.5 mM EGTA, pH 6.9) at 37℃ for 1 h. Varying concentrations (0.1 ? 125 μ M) of Combretastatin A4 are used to compete with colchicine originally bound to tubulin. After incubation, the ?ltrate is obtained. The ability of the analogue to inhibit the binding of colchicine is expressed as a percentage of control binding in the absence of any competitor.
体外活性	在NT2和MDA-MB-231乳腺肿瘤模型中,Combretastatin A4（100 mg/kg,i.p.）诱导脂质R1明显减少,并通过电子顺磁共振血氧定量法测得pO2下降. Combretastatin A4（100 mg/kg,i.p.）明显减少雄性NMRI小鼠的Ktrans.
体内活性	Combretastatin A4（1 μM）抑制35%微管蛋白聚合,10 μM几乎完全可阻断。Combretastatin A4抑制MDA-MB-231,Hela,A549,HL-60,SF295,HCT-8,MDA-MB435,OVCAR-8,PC3M,NCI-H358M和淋巴细胞的生长,IC50分别为2.8,0.9,3.8,2.1,6.2,5.3,7.9,0.37,4.7,8和3.2 nM。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : 31.6 mg/mL (100 mM) DMSO : 45 mg/mL (142.25 mM)
关键字	inhibit \| Microtubule/Tubulin \| CA 4 \| Combretastatin A4 \| Combretastatin A-4 \| CA-4 \| Inhibitor \| Combretastatin A 4
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康普瑞汀|||Z)-3,4,5,4',-四甲氧基-3'-羟基二苯乙烯|||CRC 87-09|||CA4|TargetMol

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成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子