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  • 康普瑞汀|T6212|TargetMol

康普瑞汀|T6212|TargetMol

Combretastatin A4
117048-59-6
140 20mg 起订
上海 更新日期:2024-12-02

TargetMol中国(陶术生物)

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产品详情:

中文名称:
康普瑞汀
英文名称:
Combretastatin A4
CAS号:
117048-59-6
品牌:
TargetMol
产地:
美国
保存条件:
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格:
99.81%
产品类别:
抑制剂
货号:
T6212

Product Introduction

Bioactivity

名称Combretastatin A4
描述Combretastatin A4 (CA4) is a microtubule-targeting agent that binds β-tubulin (Kd: 0.4 μM).
细胞实验MDA-MB-231, A549, and HeLa cells are grown in DMEM medium (115 units/mL of penicillin G, 115 μg/mL of streptomycin, and 10% fetal bovine serum). Cells are seeded in 96-well plates (5000 cells/well) containing 50 μL of growth medium for 24 h. After medium removal, 100 μL of fresh medium containing individual analogue compounds at di?erent concentrations is added to each well and incubated at 37 ℃ for 72 h. After 24 h of culture, the cells are supplemented with 50 μL of analogue compounds dissolved in DMSO (less than 0.25% in each preparation). After 72 h of incubation, 20 μL of resazurin is added for 2 h before recording ?uorescence at 560 nm (excitation) and 590 nm (emission) using a Victor microtiter plate ?uorimeter. The IC50 is de?ned as the compound concentration required to inhibit cell proliferation by 50% in comparison with cells treated with the maximum amount of DMSO (0.25%) and considered as 100% viability.(Only for Reference)
激酶实验Competitive binding assay using LC-MS/MS: Colchicine (1.2 μ M) is incubated with tubulin (1.3 mg/mL) in the incubation bu?er (80 mM PIPES, 2.0 mM MgCl2, 0.5 mM EGTA, pH 6.9) at 37℃ for 1 h. Varying concentrations (0.1 ? 125 μ M) of Combretastatin A4 are used to compete with colchicine originally bound to tubulin. After incubation, the ?ltrate is obtained. The ability of the analogue to inhibit the binding of colchicine is expressed as a percentage of control binding in the absence of any competitor.
体外活性在NT2和MDA-MB-231乳腺肿瘤模型中,Combretastatin A4(100 mg/kg,i.p.)诱导脂质R1明显减少,并通过电子顺磁共振血氧定量法测得pO2下降. Combretastatin A4(100 mg/kg,i.p.)明显减少雄性NMRI小鼠的Ktrans.
体内活性Combretastatin A4(1 μM)抑制35%微管蛋白聚合,10 μM几乎完全可阻断。Combretastatin A4抑制MDA-MB-231,Hela,A549,HL-60,SF295,HCT-8,MDA-MB435,OVCAR-8,PC3M,NCI-H358M和淋巴细胞的生长,IC50分别为2.8,0.9,3.8,2.1,6.2,5.3,7.9,0.37,4.7,8和3.2 nM。
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度Ethanol : 31.6 mg/mL (100 mM)
DMSO : 45 mg/mL (142.25 mM)
关键字inhibit | Microtubule/Tubulin | CA 4 | Combretastatin A4 | Combretastatin A-4 | CA-4 | Inhibitor | Combretastatin A 4
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康普瑞汀|||Z)-3,4,5,4',-四甲氧基-3'-羟基二苯乙烯|||CRC 87-09|||CA4|TargetMol

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起,为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部,化学事业部,生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应;从商业化产品销售到个性化定制合成;从对明确靶点的分子筛选到对明确分子的多靶点筛选,从高通量筛选到化学结构优化,我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘,我们已成为筛选化合物领域优秀的供应商,为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ¥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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