重组人CD8A&CD8B蛋白-ACROBiosystems百普赛斯

Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名（Synonym）

CD8 alpha & beta,CD8A & CD8B

表达区间及表达系统（Source）

Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-H52W5) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) (Accession # P01732-1 (CD8A) & P10966-1 (CD8B) ).

Predicted N-terminus: Ser 22 (CD8A) & Leu 22 (CD8B)

蛋白结构（Molecular Characterization）

Human CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 19.5 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit CD8 beta contains no tag but a basic tail at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 30-32 kDa and 32-34 kDa under reducing (R) condition, and 30-33 kDa and 58-70 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

在免疫反应中起重要作用的完整膜糖蛋白，在抵抗外部和内部攻击的反应中具有多种功能。CD8A在细胞表面与CD8B形成二硫键连接的异二聚体。CD8A同二聚体分子还促进活化淋巴细胞的存活和分化为记忆性CD8 T细胞。此外，CD8B在CD8+T细胞的胸腺选择中起着关键作用。CD8B链细胞质尾部的棕榈酰化位点有助于将CD8分配到富含信号蛋白的质膜脂筏中。