Protein G（RPG）重组蛋白-ACROBiosystems百普赛斯

Recombinant Protein G, His Tag

分子别名（Synonym）

RPG,Protein G

表达区间及表达系统（Source）

Recombinant Protein G, His Tag, also known as Recombinant Streptococcus sp. Protein G was expressed in E. coli at ACRObiosystems.

蛋白结构（Molecular Characterization）

Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>98% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Protein G是一种免疫球蛋白结合蛋白，在C组和G组链球菌中表达，与A蛋白非常相似，但具有不同的特异性。它是一种65 kDa（G148蛋白G）和58 kDa（C40蛋白G）的细胞表面蛋白，通过与Fc区结合在纯化抗体中得到了应用。天然分子也结合白蛋白，然而，由于血清白蛋白是抗体来源的主要污染物，白蛋白结合位点已从这种重组形式的Protein G中去除。