化合物 BAY 11-7082|T1902

BAY 11-7082
19542-67-7

￥185 1mg 起订

￥413 5mg 起订

￥538 10mg 起订

上海更新日期：2024-09-14

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

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邮箱：marketing@targetmol.com

产品详情:

中文名称：: 化合物 BAY 11-7082

英文名称：: BAY 11-7082

CAS号：: 19542-67-7

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.92%

产品类别：: 抑制剂

货号：: T1902

Product Introduction

Bioactivity

Name	BAY 11-7082
Description	BAY 11-7082 (BAY 11-7821) is an NF-κB inhibitor that inhibits TNFα-induced IκBα phosphorylation (IC50=10 μM). BAY 11-7082 is also an inhibitor of the ubiquitin-specific proteases USP7 and USP21 (IC50=0.19/0.96 μM).
Cell Research	Cells are transfected with siRNA in 96-well microtiter plates and then cultured for 72 hours in complete NSCLC medium, treated with BAY 11-7082 for 12 hours. Cells are incubated with [3H]thymidine for 3 hours. The cells are collected on filters using an automatic cell harvester and radioactivity on the filters is measured by β-scintillation counting.(Only for Reference)
Kinase Assay	UBE1 (0.17 μM) in 22.5 μL of 20 mM Hepes, pH 7.5, containing 10 μM ubiquitin is incubated for 45 min at 21°C with 1 μL of DMSO or 1 μL of BAY 11-7082 in DMSO. A 2.5 μL solution of 10 mM magnesium acetate and 0.2 mM ATP is added, incubated for 10 min at 30°C, and the reactions are terminated by the addition of 2.5 μL of 10% (w/v) SDS and heating for 6 min at 75°C. The samples are subjected to SDS/PAGE in the absence of any thiol. The gels are stained for 1 h with Coomassie Instant Blue and destained by washing with water. The loading of ubiquitin to E2 conjugating enzymes is carried out in an identical manner, except that UBE1 (0.17 μM) is mixed with Ubc13 (2.4 μM) or UbcH7 (2.9 μM) prior to incubation with BAY 11-7082[3].
In vitro	方法：人结直肠癌细胞 HT29 用 BAY 11-7082 (10-100 µM) 处理 1 h，随后用 TNF (50 ng/mL) 刺激 3-10 min 或用 Flag-TWEAK (200 ng/mL) 处理 8 h，使用 Western Blot 检测靶点蛋白表达水平。结果：Bay 11-7082 抑制 TWEAK 诱导的 p100 加工和 TNF 诱导的 IκBα 的磷酸化和降解。在 Bay 11-7082 的浓度为 30-100 µM 时，两种 NFκB 途径均被显著阻断。[1] 方法：巨噬细胞 RAW264.7 用 BAY 11-7082 (15 µM) 和 LPS (1 µg/mL) 处理 6 h，使用 ELISA Assay 检测 TNF-α 水平。结果：BAY 11-7082 阻断了 LPS 处理的 RAW264.7 细胞中 TNF-α 的产生，这是由活化的 NF-κB 产生的炎症反应。[2]
In vivo	方法：为研究免疫调节作用，将 BAY 11-7082 (5-10 mg/kg) 腹腔注射给 C57BL/6 小鼠，1 h 后静脉注射 poly U (50 µg/head) + in vivo-jetPEI。结果：当 TLR 配体刺激时，用 BAY 11-7082 处理可以通过限制 pDC 功能来抑制体内 IFN 反应。[3] 方法：为研究对银屑病样皮炎的作用，将 BAY 11-7082 (20 mg/kg) 腹腔注射给 IMQ 诱导银屑病样病变的 C57BL/6 和 NLRP3 KO 小鼠，每天一次，持续七天。结果：BAY 11-7082 钝化表皮厚度、棘皮病和炎症浸润。BAY 11-7082 降低了 pNF-κB、NLRP3、TNF-α、IL-6 和 IL-1β 的表达，减弱了信号转导子和 STAT3 的磷酸化，并降低了 IL-23 水平。[4]
Storage	store at low temperature,keep away from direct sunlight,store under nitrogen \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	Ethanol : 5.2 mg/mL (25 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2.07 mg/mL (9.99 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO : 60 mg/mL (289.51 mM)
Keywords	IκB kinase \| cytokine \| BAY 11-7082 \| BAY 11 7082 \| Inhibitor \| Apoptosis \| phosphorylation \| adhesion \| Deubiquitinase \| B-cell \| DUBs \| IKK \| BAY 117082 \| lymphoma \| leukaemic \| inhibit \| ubiquitin \| I kappa B kinase \| inflammasome \| Autophagy \| T-cell
Inhibitors Related	Guanidine hydrochloride \| Naringin \| Taurine \| Gefitinib \| Hydroxychloroquine \| 5-Fluorouracil \| Curcumin \| Stavudine \| Tributyrin \| L-Ascorbic acid \| Paeonol \| Sodium 4-phenylbutyrate
Related Compound Libraries	Inhibitor Library \| CNS-Penetrant Compound Library \| Pyroptosis Compound Library \| Bioactive Compound Library \| Anti-Cancer Compound Library \| Bioactive Compounds Library Max \| Kinase Inhibitor Library \| Anti-Aging Compound Library \| Anti-Liver Cancer Compound Library \| Anti-Prostate Cancer Compound Library

BAY 11-7821|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子