3-甲基腺嘌呤|T1879

3-Methyladenine
5142-23-4

￥449 50mg 起订

￥668 100mg 起订

￥1160 200mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

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产品详情:

中文名称：: 3-甲基腺嘌呤

英文名称：: 3-Methyladenine

CAS号：: 5142-23-4

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature,keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | Shipping with blue ice.

纯度规格：: 99.51%

产品类别：: 抑制剂

货号：: T1879

Product Introduction

Bioactivity

名称	3-Methyladenine
描述	3-Methyladenine (3-MA) is a PI3K inhibitor that selectively inhibits class IB PI3Kγ (IC50=60 μM) and class III VPS34 (IC50=25 μM). 3-Methyladenine inhibits autophagy.
细胞实验	Cells were seeded in an 8-well coverglass-bottomed chamber for 24 hours (6×10^3 cells per well). Images were acquired automatically at multiple locations on the coverglass using a Nikon TE2000E inverted microscope fitted with a 20× Nikon Plan Apo objective, a linearly-encoded stage, and a Hamamatsu Orca-ER CCD camera. A mercury-arc lamp with two neutral density filters (for a total 128-fold reduction in intensity) was used for fluorescence illumination. The microscope was controlled using NIS-Elements Advanced Research software and housed in a custom-designed 37°C chamber with a secondary internal chamber that delivered humidified 5% CO2. Fluorescence and differential interference contrast images were obtained every 10 min for a period of 48 hours. To analyze live cell imaging movies, the time-lapse records of live cell imaging experiments were exported as an image series and analyzed manually using NIS-Elements Advanced Research software. The criteria for analyses were described previously, and lagging chromosomes in prometaphase were defined as the red fluorescence-positive materials that lingered outside the roughly formed metaphase plate for more than 3 frames (30 min) [2].
动物实验	All rats were fasted for 12 h with free access to water prior to operation. After anesthesia by intraperitoneal (i.p.) injection of 2% sodium pentobarbital (0.25 mL/100 g), they were laid and fixed on the table, routinely shaven, disinfected, and draped. The rat SAP model was induced by 0.1 mL/min speed uniformly retrograde infusion of a freshly prepared 3.5% sodium taurocholate solution (0.1 mL/100 g) into the biliopancreatic duct after laparotomy. Equivalent volume of normal saline solution was substituted for 3.5% sodium taurocholate solution in the sham-operation (SO) control group. The incision was closed with a continuous 3-0-silk suture, and 2 mL/100 g of saline was injected into the back subcutaneously to compensate for the fluid loss. 180 rats were randomly divided into four groups: (1) Acanthopanax treatment group (Aca group, n = 45) where the rats were injected with 0.2% Acanthopanax injection at a dose of 3.5 mg/100 g 3 h after successful modeling via the vena caudalis once, knowing that this dosage was effective as proven in our previous experiment; (2) 3-Methyladenine treatment group (3-methyladenine group, n = 45) where the rats were injected with 100 nmol/μL 3-methyladenine solution at a dose of 1.5 mg/100 g 3 h after successful modeling via the intraperitoneal route once, knowing that this dosage was effective as proven in the literature [6]; (3) SAP model group (SAP group, n = 45) where these rats received an equivalent volume of the normal saline instead of Acanthopanax injection 3 h after successful modeling via the vena caudalis once; (4) SO group (control, n = 45) where these rats received an equivalent volume of the normal saline instead of Acanthopanax injection 3 h after successful sham-operation via the vena caudalis once. The 45 animals in each of the four groups were equally randomized into 3, 12, and 24 h subgroups for postoperative observations [4].
体外活性	方法：人宫颈癌细胞 HeLa 用 3-Methyladenine (2.5-10 mM) 处理 48 h，使用 Trypan blue dye exclusion assay 检测细胞生长抑制情况。结果：3-Methyladenine 以时间和剂量依赖的方式降低 HeLa 细胞活力。[1] 方法：脂肪细胞 3T3-L1 在没有血清的情况下用 3-Methyladenine (5 mM) 处理 4 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果：3-Methyladenine 显著降低了自噬标记物 LC3-II 的细胞内水平，增加了 p62 的表达，表明 3-Methyladenine 有效抑制自噬。[2] 方法：小鼠黑色素瘤细胞 B16 用 2DG (5 mM)、rotenone (1 μM) 和 3-Methyladenine (1.2-5 mM) 处理 24 h，使用 LDH release assay 检测细胞毒性。结果：3-Methyladenine 剂量依赖性降低 2DG/rotenone 引起的 LDH 释放上调，保护肿瘤细胞免受糖酵解和线粒体呼吸抑制。[3]
体内活性	方法：为研究 3-Methyladenine 对动脉粥样硬化的影响，将 3-Methyladenine (30 mg/kg) 腹腔注射给 HFD 喂养的 ApoE−/− 小鼠，每周两次，持续八周。结果：在高脂肪饮食喂养的小鼠中， 3-Methyladenine 治疗显著减少了动脉粥样硬化斑块的大小，并增加了病变的稳定性。3-Methyladenine 具有多种动脉粥样硬化保护作用，包括调节巨噬细胞自噬和泡沫细胞形成以及改变免疫微环境。[4] 方法：为研究自噬的调节作用，将 3-Methyladenine (15 mg/kg ) 单剂量腹腔注射给 LPS 诱导内毒素休克的 C57/BL6 小鼠。结果： LPS 联合 3-Methyladenine 治疗的动物在内毒素血症后表现出存活率增加，血清炎症介质 TNF-α 和 IL-6 降低。[5]
存储条件	store at low temperature,keep away from direct sunlight,keep away from moisture \| Powder: -20°C for 3 years \| Shipping with blue ice.
溶解度	DMSO : 13.75 mg/mL (92.19 mM), Heating is recommended.(The compound is unstable in solution, please use soon.) Ethanol : 4 mg/mL (26.81 mM) H2O : 3 mg/mL (20.11 mM）, Sonication and heating are recommended. (The compound is unstable in solution, please use soon.)
关键字	3 Methyladenine \| inhibit \| Mitochondrial Autophagy \| 3Methyladenine \| NSC66389 \| PI3K \| Endogenous Metabolite \| 3-Methyladenine \| Mitophagy \| Autophagy \| NSC-66389 \| Phosphoinositide 3-kinase \| Inhibitor
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NSC 66389|||3-甲基腺嘌呤|||3-MA|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子