硫酸博来霉素|T6116

Bleomycin Sulfate
9041-93-4

￥287 1mg 起订

￥397 2mg 起订

￥672 5mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 硫酸博来霉素

英文名称：: Bleomycin Sulfate

CAS号：: 9041-93-4

品牌：: TargetMol

产地：: 美国

保存条件：: keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: ≥98%

产品类别：: 抑制剂

货号：: T6116

Product Introduction

Bioactivity

名称	Bleomycin Sulfate
描述	Bleomycin Sulfate (Blenoxane) is a glycopeptide antibiotic, an inhibitor of DNA synthesis. Bleomycin Sulfate causes DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has antitumor activity.
细胞实验	ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)
体外活性	方法：人胰腺细胞 (PCCs) AsPC-1 和 MIA PaCa-2 用 Bleomycin Sulfate (0.1-100 µM) 处理 24-72 h，使用 MTT 方法检测细胞生长抑制情况。结果：AsPC-1 细胞对 Bleomycin Sulfate 无反应。Bleomycin Sulfate 显著抑制了 MIA-PaCa-2 的细胞生长，在处理 24/48/72 h 时的 IC50 分别为 5.9/6.4/2.6 μM。[1] 方法：间充质干细胞 MSCs 用 Bleomycin Sulfate (1800 ng/mL) 处理 24-96 h，使用 Flow Cytometry 方法检测 sub-G1 和 caspase-3。结果：Bleomycin Sulfate 诱导 sub-G1 和 caspase-3 的激活上调，表明 Bleomycin Sulfate 诱导细胞凋亡。[2] 方法：正常大鼠肺泡巨噬细胞用 Bleomycin Sulfate (0.01-1 μg/mL) 孵育 18 h，使用 MDGF Assay 测定其水平。结果：Bleomycin Sulfate 刺激巨噬细胞产生成纤维细胞生长因子 (MDGF)。[3]
体内活性	方法：为检测体内抗肿瘤活性，将 Bleomycin Sulfate (20 mg/kg) 腹腔注射给携带小鼠结直肠癌瘤肿瘤 CT26 shControl 或 shCRT 的 BALB/c 小鼠，每两天一次，给药五次。结果：Bleomycin Sulfate 延迟了对照 shRNA 转染的 CT26 细胞的生长，但对 CRT shRNA 转染 CT26 细胞没有影响。[4] 方法：为研究博来霉素诱导的小鼠肺纤维化的时间过程，将 Bleomycin Sulfate (0.06 mg/只 in 0.9% saline) 单次气管内滴注 (IT) 给 C57Bl/6J 小鼠。结果：Bleomycin Sulfate 诱导小鼠肺纤维化。该模型中评估肺纤维化的最合适的时间点是在 IT 滴注 Bleomycin Sulfate 后 14 天，这是基于观察到的动物在 14 天时出现广泛的纤维化，但纤维化反应的变异性较小，死亡率低于21天。[5]
存储条件	keep away from moisture,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : < 1 mg/mL (insoluble or slightly soluble) H2O : 92 mg/mL (60.8 mM) DMSO : 93 mg/mL (61.5 mM)
关键字	inhibit \| NSC 125066 \| DNA/RNA Synthesis \| Antibiotic \| Bleomycin \| NSC-125066 \| Inhibitor \| Bleomycin Sulfate
相关产品	Cefaclor monohydrate \| Guanidine hydrochloride \| G-418 disulfate \| Doxycycline \| Neomycin sulfate \| Metronidazole \| 5-Fluorouracil \| EDTA copper(II) disodium salt \| Ampicillin sodium \| Thymidine \| Sulfamethoxazole sodium \| Kanamycin sulfate
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硫酸博莱霉素|||硫酸博来霉素|||NSC125066|||Blenoxane|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子