3,4-二羟基苯甲酸|T0562

Protocatechuic acid
99-50-3

￥333 200mg 起订

上海更新日期：2024-12-02

TargetMol中国（陶术生物）

VIP12年

联系人：邵小姐

电话：021-021-33632979拨打

手机：15002134094 拨打

邮箱：marketing@targetmol.com

产品详情:

中文名称：: 3,4-二羟基苯甲酸

英文名称：: Protocatechuic acid

CAS号：: 99-50-3

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.85%

产品类别：: 抑制剂

货号：: T0562

Product Introduction

Bioactivity

名称	Protocatechuic acid
描述	Protocatechuic acid (3, 4-Dihydroxybenzoic acid) (3, 4-dihydroxybenzoic acid) is a natural phenolic compound found in many edible and medicinal plants. Recent studies indicate that it could be used as a protective agent against cardiovascular diseases and neoplasms. The mechanism of its action is mostly associated with antioxidant activity, including inhibition of generation as well as scavenging of free radicals and up-regulating enzymes which participate in their neutralization.
细胞实验	Protocatechuic acid is dissolved in DMSO. Dilutions of Protocatechuic acid (2, 5, 10, 20, 50, and 100 μM) are prepared from stock solutions, with serum-free culture medium. Equal volumes of each solution are mixed with Aβ1-42 (10 μM), then incubated for 24 h on a thermoblock, with continuous agitation, and then exposed to PC12 cells for 24 h to test whether Protocatechuic acid can prevent cell death triggered by Aβ. Cell viability is determined by MTT reduction assay. Cells are treated with 200 μL per well of MTT solution (final concentration, 0.5 mg/mL in DMEM-Glutamax medium) for 3 h, at 37°C, with 5% CO2. The dark blue formazan crystals that formed are solubilized with 100 μL per well of DMSO, for 30 min. Absorbance is measured at 540 nm, with a microplate reader. Results are expressed as the percentage of MTT reduction in relation to the absorbance of control cells at 100%.
激酶实验	AChE activity investigation is carried out in a reaction mixture containing 50 μL of tissue homogenate, 50 μL of 5, 5'-dithiobis-(2-nitrobenzoic) acid (DTNB), 1175 μL of 0.1 M phosphate-buffered solution, pH 8.0. After incubation for 20 min at 25°C, 25 μL of acetylthiocholine iodide solution is added as the substrate. The AChE activity is determined as changes in absorbance reading at 412 nm for 3 min at 25°C and using a UV/Visible spectrophotometer.
体外活性	Protocatechuic acid 抑制Aβ和αS的聚集，破坏其已形成的纤维结构，预防由Aβ和αS诱导的毒性引起的PC12细胞死亡[3]。
体内活性	Protocatechuic acid 能在不改变小鼠运动活动的情况下，防止应激诱导的强迫游泳试验中的静止时间。此外，Protocatechuic acid 还能减轻 ARS 小鼠血清皮质酮的升高和脂质过氧化反应，并恢复大脑皮层和海马中的酶抗氧化剂[1]。给大鼠注射镉并用前列腺素和Protocatechuic acid （10-20 mg/kg）治疗后，其 BChE 活性明显降低。镉和前列腺素或Protocatechuic acid （10-20 mg/kg）处理大鼠后，MDA 水平明显下降[2]。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : 5 mg/mL (32.44 mM), Sonication is recommended. DMSO : 55 mg/mL (356.86 mM)
关键字	Endogenous Metabolite \| Inhibitor \| Protocatechuic acid \| inhibit
相关库	神经保护化合物库 \| 微生物天然产物库 \| 经典已知活性库 \| 植物来源化合物库 \| 古代经典名方目录分子库 \| 苗药化合物库 \| 药食同源库 \| 中药单体化合物库 \| 天然产物库 \| 中药抗炎分子库

原儿茶酸|||Protocatechuate|||3,4-二羟基苯甲酸|||3,4-Dihydroxybenzoic acid|||3, 4-Dihydroxybenzoic acid|TargetMol

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子