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网站主页 化工产品目录 原料药 抗肿瘤药 抗生素类抗肿瘤药 盐酸多柔比星 盐酸阿霉素
  • 盐酸阿霉素|T1020

盐酸阿霉素|T1020

Doxorubicin hydrochloride
25316-40-9
￥155 5mg 起订
￥273 10mg 起订
￥455 25mg 起订
上海 更新日期：2025-03-12

TargetMol中国（陶术生物）

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联系人：邵小姐
电话：021-021-33632979拨打
手机：15002134094 拨打
邮箱：marketing@targetmol.com

产品详情:

中文名称：
盐酸阿霉素
英文名称：
Doxorubicin hydrochloride
CAS号：
25316-40-9
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.57%
产品类别：
抑制剂
货号：
T1020

Product Introduction

Bioactivity

名称Doxorubicin hydrochloride
描述Doxorubicin hydrochloride (Adriamycin) belongs to the anthracycline class of antibiotics and is an inhibitor of human DNA topoisomerase I/II (IC50=0.8/2.67 μM). Doxorubicin hydrochloride exhibits cytotoxicity and antitumor activity. Doxorubicin hydrochloride reduces the phosphorylation of AMPK and its downstream target protein acetyl coenzyme A carboxylase, and induces apoptosis and autophagy.
细胞实验To analyze the effect of Bcl-2 expression on the viability of HUVECs treated with Dox, cells were co-transfected with 200 ng of the pEGFP-spectrin expression plasmid together with 200 ng of either pCDNA3-hBcl-2 or the control pCMVβ-galactosidase expression vector (33). The pGL3 Basic vector (2.1 μg) was added as a DNA carrier in a total volume of 0.140 ml, and transfection was performed by the calcium phosphate procedure in 35-mm tissue culture dishes. After treatment, the cells were washed with PBS, fixed with 3.7% formaldehyde for 15 min, and washed for a further 10 min with 50 mM NH4Cl blocking solution in PBS. Cells were then washed with PBS, permeabilized with a 0.1% Triton X-100 for 10 min, washed again with PBS, and stained with 1 μg/ml 4′,6-diamidino-2-phenyl-indole solution for 2 min. The cells were examined under a fluorescence microscope, and GFP-positive cells were scored after counting a minimum of 1000 total cells for each condition. The efficiency of transfection in Bcl-2- and β-galactosidase-expressing cells, determined in aliquots of transfected cells just before the addition of Dox, was similar (10–12%) [1].
动物实验Athymic male nude mice (3-4 weeks old) are used. PC3 cells (4×106) are injected subcutaneously into the flanks of mice. Animals bearing tumors are randomly assigned to treatment groups (five or six mice per group) and treatment initiated when xenografts reached volumes of about 100 mm3. Tumors are measured using digital calipers and volume calculated using the formula: Volume=Width2×Length×0.52, where width represents the shorter dimension of the tumor. Treatments are administered as indicated using vehicle (PBS containing 0.1% BSA), Doxorubicin (2-8 mg/kg), Apo2L/TRAIL (500 μg/animal), or a combination of 4 mg/kg Doxorubicin followed by 500 μg Apo2L/TRAIL. Doxorubicin is administered systemically whereas Apo2L/TRAIL is given either intratumorally or systemically. All treatments are given once. Mice are monitored daily for signs of adverse effects (listlessness and scruffy appearance). Treatments seemed to be well tolerated. The mean±SEM is calculated for each data point. Differences between treatment groups are analyzed by the student t-test. Differences are considered significant when P<0.05 [3]. Altogether, 29 male Wistar rats (weight 306 ± 18.6 g) were used in the study. Animals were divided into three groups: control (group C; n = 10; 306.4 ± 17.2 g), animals treated with DOX (group DOX; n = 10; 305.0 ± 24.9 g) and animals treated with L-DOX (group L-DOX; n = 9; 306.7 ± 15.0 g). Vehiculum (aqua pro injection), DOX and L-DOX were applied to group C, DOX and L-DOX, respectively, by single intraperitoneal injection; concentration of both DOX and L-DOX was 5 mg/kg, similar to the concentrations used in human treatment protocols. All animals were sacrificed 24 h after drug application. Thoracotomy was performed, hearts were excised and samples were obtained separately from the free wall of the left atrium (LA), left ventricle (LV), right atrium (RA) and right ventricle (RV).Samples were placed into RNA later preservation solution and stored at -80 C until further analysis [4].
体外活性方法：人乳腺癌细胞 MCF10A、BT474、MCF-7 和 T47D 用 Doxorubicin hydrochloride (0.1-10 μM) 处理 48 h，使用 MTT 方法检测细胞生长抑制情况。 结果：Doxorubicin hydrochloride 剂量依赖性地抑制 MCF10A、BT474、MCF-7 和 T47D 细胞生长，IC50 分别为 2.51 µM、1.14 µM、0.69 µM 和 8.53 µM。[1] 方法：牛主动脉内皮细胞 BAECs 和 人卵巢畸胎瘤细胞 PA-1 用 Doxorubicin (0.5 μM) 处理 1-16 h，使用 Flow Cytometry 方法检测细胞凋亡情况，使用 caspase-3 assay kit 检测 caspase-3 的活性。 结果：Doxorubicin 时间依赖性地诱导 BAECs 和 PA-1 细胞的凋亡及 caspase-3 激活。[2] 方法：犬乳腺癌细胞 CIPp 用 Doxorubicin (EC50(20h)=12.08 μM) 处理 3-48 h，使用 qRT-PCR 方法检测靶基因表达情况。 结果：Doxorubicin 诱导多药耐药性 (MDR) 相关基因 P-gp 和 BCRP 的 mRNA 表达水平上调。[3]
体内活性方法：为检测体内抗肿瘤活性，将 Doxorubicin hydrochloride (1 mg/kg/4 天) 和 lovastatin (5  mg/kg/天) 腹腔注射给携带鼠黑色素瘤肿瘤 B16F10 的 B6D2F1 小鼠，持续两周。 结果：与单独作用的任一药物相比，Doxorubicin hydrochloride 和 lovastatin 联合治疗的敏感性显著增加，lovastatin 增强了 Doxorubicin hydrochloride 的抗肿瘤活性。[4] 方法：为研究 Doxorubicin 对癌症患者的急性和长期认知障碍，将 Doxorubicin hydrochloride (25 mg/kg) 单剂量腹腔注射给 B6C3F1J 小鼠。 结果：Doxorubicin hydrochloride 全身治疗在 24 h 内改变了与认知功能相关的关键细胞核中的谷氨酸神经传递，对空间学习和记忆没有持久影响。[5]
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度DMSO : 55 mg/mL (94.83 mM)
H2O : 50 mg/mL (86.2 mM), Sonication is recommended.
关键字AMPK | Doxorubicin | Topoisomerase | Doxorubicin Hydrochloride | Human immunodeficiency virus | HBV | HIV | Mitochondrial Autophagy | Bacterial | Mitophagy | Apoptosis | Doxorubicin hydrochloride | Autophagy | Inhibitor | Hepatitis B virus | ADC Payload | ADC Cytotoxin | Hydroxydaunorubicin | inhibit | NSC-123127 | AMP-activated protein kinase | Hydroxydaunorubicin Hydrochloride | Antibiotic | NSC123127
相关产品Chitosan oligosaccharide | Guanidine hydrochloride | Naringin | Emtricitabine | Doxycycline | Neomycin sulfate | Hydroxychloroquine | Lamivudine | Dimethyl sulfoxide | Dextran sulfate sodium salt (MW 4500-5500) | Sulfamethoxazole sodium | Paeonol
相关库抗癌活性化合物库 | 经典已知活性库 | 抗癌上市药物库 | 已知活性化合物库 | 激酶抑制剂库 | EMA 上市药物库 | 抗衰老化合物库 | 中药单体化合物库 | 药物功能重定位化合物库 | 高通量筛选天然产物库
盐酸多柔比星|||盐酸阿霉素|||NSC 123127|||Hydroxydaunorubicin hydrochloride|||Doxorubicin (Adriamycin) HCl|||DOX hydrochloride|||Adriamycin|TargetMol

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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