人透明质酸酶（Hyaluronidase）PH20-ACROBiosystems百普赛斯

Human Hyaluronidase PH-20 / SPAM1 Protein, Tag Free (active enzyme, MALS verified)

表达区间及表达系统（Source）

Human Hyaluronidase PH-20 Protein, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).

Predicted N-terminus: Leu 36

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

透明质酸酶PH-20也称为精子粘附分子1（SPAM1）和精子表面蛋白PH-20，属于糖基水解酶56家族，SPAM1/PH-20在睾丸中表达。透明质酸中N-乙酰基-β-D-葡糖胺和D-葡萄糖醛酸残基之间（1->4）键的SPAM-1/PH20随机水解。SPAM-1/PH20参与精卵粘附。受精后，精子必须首先穿透卵子周围的一层卵丘细胞，然后才能到达透明带。卵丘细胞包埋在排卵前形成的含有透明质酸的基质中。SPAM1通过消化透明质酸帮助穿透卵丘细胞层。