磷酸氯喹|T0194

Chloroquine phosphate
50-63-5

￥298.00 50瓶起订

￥1270.00 500瓶起订

￥418.00 100瓶起订

￥656.00 200瓶起订

上海更新日期：2024-09-14

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 磷酸氯喹

英文名称：: Chloroquine phosphate

CAS号：: 50-63-5

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.79%

产品类别：: 抑制剂

货号：: T0194

Product Introduction

Bioactivity

名称	Chloroquine phosphate
描述	Chloroquine phosphate (Aralen phosphate) is an aminoquinoline antimalarial and also is widely used as an autophagy inhibitor. Chloroquine also is an inhibitor of toll-like receptors (TLRs).
细胞实验	The cells are cultured in 6-well plates with normal culture medium in the presence of vehicle or 25 or 50 μM chloroquine, until near confluency, after which they are rinsed with sterile phosphate-buffered saline (PBS) and cultured further for the indicated times in serum-free culture medium. At the desired time-points, the culture medium is discarded and the cells are quickly harvested in lysis buffer and clarified by centrifugation. Subsequent to boiling the supernatants in reducing sodium dodecyl sulfate (SDS) sample buffer, equal amounts of protein (100 μg) are loaded per lane and the samples are electrophoresed into 10 or 4-20% gradient polyacrylamide SDS gels, then transferred to a nitrocellulose membrane. To detect TLR9, the blots were incubated overnight at 4°C with anti-TLR9 antibodies, diluted 1:500 in Tris-buffered saline with 0.1% (v/v) Tween-20 (TBST). Equal loading is confirmed with polyclonal rabbit anti-actin. Secondary detection is performed with horseradish peroxidase-linked secondary antibodies. The protein bands are visualized by chemiluminescence using an ECL kit [2].
动物实验	Control and TLR9 siRNA MDA-MB-231 cells (5×105 cells in 100 μL) are inoculated into the mammary fat pads of four-week-old, immune-deficient mice (athymic nude/nu Foxn1). Treatments are started seven days after tumor cell inoculation. The mice are treated daily either with intraperitoneal (i.p.) chloroquine (80 mg/kg) or vehicle (PBS). The animals are monitored daily for clinical signs. Tumor measurements are performed twice a week and tumor volume is calculated according to the formula V=(π/6) (d1×d2)3/2, where d1 and d2 are perpendicular tumor diameters. The tumors are allowed to grow for 22 days, at which point the mice are sacrificed and the tumors are dissected for a final measurement. Throughout the experiments, the animals are maintained under controlled pathogen-free environmental conditions (20-21°C, 30-60% relative humidity and a 12-h lighting cycle). The mice are fed with small-animal food pellets and supplied with sterile water ad libitum [2].
体外活性	Chloroquine （20 μM）可抑制 IL-12p70 的释放，并降低活化的人单核细胞衍生的朗格汉斯样细胞（MoLC）的 Th1 诱导能力。Chloroquine （20 μM）可增强 IL-1 诱导的 MoLC IL-23 分泌，并随后增加启动的 CD4+ T 细胞的 IL-17A 释放[1]。Chloroquine （25 μM）可抑制亲代 MDA-MB-231 细胞在常氧和缺氧情况下的 MMP-9 mRNA 表达。Chloroquine 对 MMP-2、MMP-9 和 MMP-13 mRNA 的表达具有细胞、剂量和缺氧依赖性影响[2]。使用Chloroquine 抑制 TLR7 和 TLR9 可显著减少体外 HuH7 细胞的增殖[3]。
体内活性	在正位小鼠模型中，Chloroquine （80 mg/kg，静脉注射）不能阻止 TLR9 表达水平高或低的三阴性 MDA-MB-231 细胞的生长[2]。使用Chloroquine 抑制 TLR7 和 TLR9 可显著抑制小鼠异种移植模型中肿瘤的生长。Chloroquine 也能显著抑制 DEN/NMOR 大鼠模型中 HCC 的发展 [3]。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : Insoluble H2O : 10 mM
关键字	inhibit \| Chingamin Phosphate \| COVID-19 \| Chloroquine phosphate \| Chloroquine \| Autophagy \| Inhibitor \| rheumatoid \| immune-modulating \| Toll-like Receptor (TLR) \| Antibiotic \| HIV \| SARS coronavirus \| Chloroquine Phosphate \| infection \| Parasite \| Human immunodeficiency virus \| arthritis \| Chloroquine Diphosphate \| malaria \| inflammatory \| SARS-CoV \| Aralen Phosphate
相关产品	Emtricitabine \| Doxycycline \| Neomycin sulfate \| Hydroxychloroquine \| Lamivudine \| Sulfamethoxazole sodium
相关库	抑制剂库 \| 经典已知活性库 \| 已知活性化合物库 \| 生物碱类天然产物库 \| 抗癌天然产物库 \| 天然产物库 \| 抗病毒库 \| 膜蛋白靶向化合物库 \| 抗寄生虫天然产物库 \| 抗癌药物库

Chloroquine diphosphate|||Aralen phosphate|||Chingamin phosphate|||磷酸氯喹

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期	(12年)
注册资本	566.2651万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,试剂,定制,服务
主营行业	化学试剂,生物活性小分子