重组人CD155 / PVR蛋白-ACROBiosystems百普赛斯

Human CD155 / PVR Protein, His Tag (MALS verified)

分子别名（Synonym）

PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

表达区间及表达系统（Source）

Human CD155, His Tag (CD5-H5223) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).

Predicted N-terminus: Trp 21

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CD155是免疫球蛋白超家族中的I型跨膜糖蛋白。CD155通常被称为脊髓灰质炎病毒受体（PVR），因为它参与灵长类动物的细胞脊髓灰质炎病毒感染，CD155的正常细胞功能是在上皮细胞之间建立细胞间粘附连接。CD155/PVR最初是基于其介导脊髓灰质炎病毒附着到宿主细胞的能力而分离的。全长（或CD155α亚型）合成为417个氨基酸（aa）的前体，含有20个aa的信号序列、323个aa的细胞外区域、24个aa的TM片段和50个aa的细胞质尾部。胞外区包含一个N端V型和两个C2型Ig样结构域。CD155是一种跨膜蛋白，具有3个细胞外免疫球蛋白样结构域D1-D3，其中D1被病毒识别。使用电子显微镜获得了与脊髓灰质炎病毒复合的CD155的低分辨率结构，而通过x射线晶体学解决了CD155的结构域D1和D2的高分辨率结构。