重组人gp130/CD130蛋白-ACROBiosystems百普赛斯

Human gp130 / CD130 / IL-6 R beta Protein, His Tag (MALS verified)

表达区间及表达系统（Source）

Human gp130, His Tag (ILT-H52H2) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 (Accession # P40189-1).

Predicted N-terminus: Glu 23

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 69.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-6受体亚基β（IL6ST）也称为IL-6受体亚基α、IL-6R亚基β、IL-6Rβ、IL-6RB、白细胞介蛋白-6信号转导子、膜糖蛋白130（gp130）、CD130、Oncostatin-M受体亚基a和IL6ST，后者是单程I型膜蛋白。IL6ST/gp130/CD130可以在脑、心脏、胸腺、脾脏、肾脏、肺和肝脏等组织中发现，并且在除BaF-B03之外的所有测试细胞系中都有发现。IL-6ST/gp130的表达不限于IL6反应细胞。IL6、LIF、OSM、CNTF、IL11、CTF1和BSF3的受体系统可以利用gp130启动信号传输。IL6ST/CD130可以与IL6/IL6R（α链）复合物结合，形成高亲和力IL6结合位点，并转导信号。IL6ST/GP130不结合IL6，可能在胚胎发育中发挥作用。