重组人整合素Integrin αvβ8蛋白-ACROBiosystems百普赛斯

Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free

分子别名（Synonym）

Integrin alpha V beta 8,ITGAV&ITGB8

表达区间及表达系统（Source）

Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-H52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201.1 (ITGAV) & NP_002205.1 (ITGB8)).

Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)

蛋白结构（Molecular Characterization）

Human ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.9 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

整合素αVβ8（ITGAV和ITGB8或ITGAVB8）在卵黄囊、胎盘、脑血管周围星形胶质细胞、雪旺氏细胞、肾小球系膜细胞和肺上皮细胞中表达。与其他αV整合素不同，ITGAVB8似乎没有呈现不同的激活状态，细胞质尾部也没有连接到细胞骨架。它结合含有RGD基序的配体，包括玻连蛋白、纤维蛋白和潜在TGF-β复合物的潜伏相关肽（LAP）。αVβ8与LAP的高亲和力结合允许MT1-MMP进行蛋白水解切割，从而释放活性TGF-β。这种机制不同于αVβ6，另一种αV整合素可以通过非蛋白水解机制从潜伏期激活TGF-β。TGF-β激活的下游效应包括控制细胞生长和相关的血管化。