重组人CD8 alpha/CD8A蛋白-ACROBiosystems百普赛斯

Biotinylated Human CD8 alpha / CD8A Protein, His,Avitag™

分子别名（Synonym）

CD8A,CD8,Leu2,MAL,p32

表达区间及表达系统（Source）

Biotinylated Human CD8 alpha, His,Avitag (CDA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).

Predicted N-terminus: Ser 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 21.2 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

在免疫反应中起重要作用的完整膜糖蛋白，在抵抗外部和内部攻击的反应中具有多种功能。同时与T细胞受体（TCR）和抗原呈递细胞（APC）呈递的MHC I类蛋白相互作用。反过来，Src激酶LCK被募集到TCR-CD3复合物附近。在NK细胞中，细胞表面CD8A同源二聚体的存在提供了一种存活机制，允许多个靶细胞的结合和裂解。CD8A同二聚体分子还促进活化淋巴细胞的存活和分化为记忆性CD8 T细胞。