重组人CD27 Ligand / CD70蛋白-ACROBiosystems百普赛斯

Human CD27 Ligand / CD70 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名（Synonym）

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表达区间及表达系统（Source）

Human CD27 Ligand Protein, His,Flag Tag (CDL-H52Da) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1 ) trimer Design.

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

分化簇70（CD70）也称为CD27配体（CD27L/CD27LG）、TNFSF7、TNFSF7G，是属于TNF超家族（TNFSF）的II型跨膜糖蛋白，是活化的T和B淋巴细胞以及成熟树突状细胞上发现的表面抗原。CD70与其受体CD27的结合诱导了T细胞的启动、效应器功能、分化和记忆形成，因此参与了包括T细胞活化、共刺激T细胞增殖以及细胞溶解性T细胞生成在内的生物过程。T细胞上的CD70提供T细胞增殖、克隆扩增和促进效应T细胞形成所需的共刺激信号。小鼠B细胞上的CD70已被证明可以抑制活化的B细胞向浆细胞的终末分化，并增强对记忆B细胞反应的承诺。CD70诱导NK细胞增殖和IFNγ产生。