食蟹猴CD117蛋白-ACROBiosystems百普赛斯

Cynomolgus CD117 / c-kit Protein, His Tag (MALS verified)

分子别名（Synonym）

CD117,SCFR,c-Kit,KIT

表达区间及表达系统（Source）

Cynomolgus CD117, His Tag (CD7-C52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 521 (Accession # XP_005555329.1).

Predicted N-terminus: Gln 26

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 57.6 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Mast/干细胞生长因子受体试剂盒（c-Kit）是蛋白激酶超家族、Tyr蛋白激酶家族和CSF-1/PDF受体亚家族的成员，也被称为花斑性状蛋白（PBT）、p145 c-Kit、酪氨酸蛋白激酶试剂盒和CD117，其中包含五个Ig样C2型（免疫球蛋白样）结构域和一个蛋白激酶结构域。CD117作为细胞因子KITLG/SCF的细胞表面受体，在调节细胞存活和增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和功能以及黑色素生成中起着至关重要的作用。作为对KITLG/SCF结合的反应，CD117可以激活多种信号通路。此外，活化的KIT促进蛋白磷酸酶PTPN6/SHP-1和PTPRU以及转录因子STAT1、STAT3、STAT5A和STAT5B的磷酸化。