重组人LILRB1蛋白-ACROBiosystems百普赛斯

Biotinylated Human LILRB1 / CD85j / ILT2 Protein, Fc,Avitag™

分子别名（Synonym）

CD85J,LILRB1,CD85,ILT2,LIR1,MIR7

表达区间及表达系统（Source）

Biotinylated Human LILRB1, Fc,Avitag (CDJ-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - His 458 (Accession # D9IDM8-1).

Predicted N-terminus: Gly 24

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 75.6 kDa. The protein migrates as 100-116 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

LILRB1（Uniprot No.D9IDM8-1）是一种与NK细胞上LILRB1低表达相关的变体。它反映了LILRB1基因座的多样性及其对NK细胞表达模式的影响。LILRB1属于LIR受体的B亚家族，其包含两个或四个细胞外免疫球蛋白结构域、一个跨膜结构域和两到四个细胞质免疫受体酪氨酸基抑制基序（ITIM）。受体在免疫细胞上表达，与抗原呈递细胞上的MHC I类分子结合，并传递抑制免疫反应刺激的负信号。它被认为可以控制炎症反应和细胞毒性，以帮助集中免疫反应并限制自身反应性。