重组人GPRC5D蛋白-ACROBiosystems百普赛斯

Biotinylated Human GPRC5D Protein, His,Avitag™&Flag Tag (Detergent)

分子别名（Synonym）

GPRC5D

表达区间及表达系统（Source）

Biotinylated Human GPRC5D, His,Avitag&Flag Tag (GPD-H82D6) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # Q9NZD1-2).

Predicted N-terminus: Asp

蛋白结构（Molecular Characterization）

This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).

The protein has a calculated MW of 53.5 kDa. The protein migrates as 35-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>80% as determined by SDS-PAGE.

制剂（Formulation）

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

G蛋白偶联受体家族C组5成员D（GPRC5D）是一种维甲酸诱导的40kDa蛋白，具有七个跨膜片段和一个短的N端细胞外区。在外围系统中广泛表达。表达模式在胰腺中较高，在肾脏、小肠、脾脏和睾丸中中等，在肺、结肠、白细胞、前列腺和胸腺中较低，在脑、心脏、肝脏、胎盘、骨骼肌和卵巢中未检测到。