重组人LILRB2蛋白-ACROBiosystems百普赛斯

Biotinylated Human LILRB2 / CD85d / ILT4 Protein, Fc,Avitag™ (MALS verified)

分子别名（Synonym）

LILRB2,ILT4,LIR2,MIR10,CD85d

表达区间及表达系统（Source）

Biotinylated Human LILRB2, Fc,Avitag (LI2-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # AAH36827.1).

Predicted N-terminus: Gln 22

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 76.2 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞免疫球蛋白样受体亚家族B成员2（LILRB2）也称为CD85抗原样家族成员D（CD85d）、免疫球蛋白类转录物4（ILT-4）、单核细胞/巨噬细胞免疫球蛋白状受体10（MIR-10），它是LIR受体亚家族的B类成员。LILRB2是I类MHC抗原的受体。LILRB2识别广泛的HLA-a、HLA-B、HLA-C和HLA-G等位基因。LILRB2与CD8A竞争结合I类MHC抗原。LILRB2/CD85d抑制FCGR1A介导的细胞蛋白磷酸化和细胞内钙离子的动员。