重组人Siglec-10蛋白-ACROBiosystems百普赛斯

PE-Labeled Human Siglec-10 Protein, His Tag (recommended for neutralizing assay) (Site-specific conjugation)

分子别名（Synonym）

SIGLEC10,MGC126774,PRO940,Siglec10,SLG2

表达区间及表达系统（Source）

PE-Labeled Human Siglec-10, His Tag (SI0-HP2E5) is produced via site-specific conjugation of PE to Human Siglec-10, His Tag under optimal conditions with a proprietary technology with a proprietary technology. Human Siglec-10, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

Predicted N-terminus: Met 17

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 61.8 kDa.

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

应用说明（Application）

Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.

Designed and validated to use together with CD24 overexpressing HEK293 cells.

Not suitable to use together with overexpressing CHO cells for high non-specific background from proteins of high sialylation.

Not recommended and validated to use together with tumor cells.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

Siglecs（唾液酸结合Ig样凝集素）是具有粘附分子样结构的Ig超家族的一个独特子集。我们在这里描述了siglec蛋白家族的一个新成员，它具有相似的结构，包括五个Ig样结构域、一个跨膜结构域和一个含有两个ITIM信号基序的细胞质尾部。Siglec-10是通过哮喘嗜酸性粒细胞EST库的数据库挖掘鉴定的。Siglec-10-VAP-1相互作用似乎介导淋巴细胞与内皮细胞的粘附，并有可能通过酶终产物改变炎症微环境。