重组人DLL4蛋白-ACROBiosystems百普赛斯

Biotinylated Human DLL4 Protein, His,Avitag™, premium grade

分子别名（Synonym）

DLL4,Delta4

表达区间及表达系统（Source）

Biotinylated Human DLL4, His,Avitag, premium grade (DL4-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # NP_061947.1).

Predicted N-terminus: Ser 27

该产品是在我们严格的质量控制体系下生产的，该体系包含一套全面的测试，包括无菌和内毒素测试。在早期临床前阶段，产品性能经过仔细验证和测试，以确保其与细胞培养用途或任何其他应用的兼容性。当准备过渡到后期临床阶段时，我们还提供定制的GMP蛋白质服务，以满足您的需求。我们将与您合作，根据您的要求定制和开发GMP级产品，该产品也符合细胞疗法细胞制造中使用的原材料和辅助材料的要求。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 57.9 kDa. The protein migrates as 61 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotinylation）

As determined by Quantative ELISA binding assay against streptavidin.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

德尔塔样蛋白4（DLL4）也被称为果蝇德尔塔同源物4（Delta4），它包含一个DSL结构域和八个EGF样结构域。DLL4在血管内皮中表达。DLL4作为Notch配体参与Notch信号通路，可以激活NOTCH1和NOTCH4。DLL4参与血管生成，并负调控内皮细胞增殖和迁移以及血管生成发芽。DLL4可以与Notch-1和Notch-4结合。