重组人TFPI蛋白-ACROBiosystems百普赛斯

Human TFPI Protein, His Tag (active enzyme)

分子别名（Synonym）

TFPI,LACI,TFPI1,EPI,TFI

表达区间及表达系统（Source）

Human TFPI, His Tag (TFI-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).

Predicted N-terminus: Asp 29

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

组织因子途径抑制物（TFPI）也称为外源途径抑制物（EPI）、脂蛋白相关凝血抑制物（LACI），是一种由血管内皮细胞合成的血浆蛋白酶抑制物，其中一部分与这些细胞的糖胺聚糖有关。TFPI是一种单链多肽，可以可逆地抑制因子Xa（Xa）和凝血酶（因子IIa）。TFPI是一种分泌蛋白，具有N端酸性区、由两个接头区分隔的三个Kunitz（K）结构域和一个C端碱性区。第一个K结构域抑制与组织因子（TF）复合的凝血因子VIIa；第二个K结构域抑制因子Xa；第三个K结构域与肝素结合；C终端基本区域可以具有多种功能。例如，它在TFPI与细胞表面的结合中起着重要作用。