重组人B7-1/CD80蛋白-ACROBiosystems百普赛斯

Human B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

分子别名（Synonym）

CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7

表达区间及表达系统（Source）

Human B7-1, Mouse IgG2a Fc Tag (B71-H52A4) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Predicted N-terminus: Val 35

蛋白结构（Molecular Characterization）

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

B7-1和B7-2及其受体CD28和CTLA4共同构成了调节T和B细胞反应的主要共刺激途径之一。尽管CTLA 4和CD28都可以结合相同的配体，但CTLA 4结合B7 1和B7 2的亲和力比CD28高20-100倍，并参与免疫反应的下调。

B淋巴细胞活化抗原B7-1（简称B7），也称为分化簇80（CD80），是细胞表面免疫球蛋白超家族的成员，在活化的B细胞、活化的T细胞、巨噬细胞和树突状细胞上表达。它是T细胞表面两种不同蛋白质的配体：CD28（用于自动调节和细胞间结合）和CTLA-4（用于减弱调节和细胞分离）。CD80与CD86协同作用以激活T细胞。CD80通过激活巨噬细胞中的NF-κB信号通路在诱导先天免疫反应中发挥作用。因此，CD80被认为是治疗自身免疫性疾病和各种癌症的有前景的靶点。