重组人Clusterin/CLUS蛋白-ACROBiosystems百普赛斯

Human Clusterin / CLUS Protein, His Tag

分子别名（Synonym）

CLU,Clusterin,AAG4,APOJ,CLI,KUB1,SGP-2,SP-40,TRPM2

表达区间及表达系统（Source）

Human Clusterin, His Tag (CLU-H5227) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Glu 449 (Accession # NP_001822.3).

Predicted N-terminus: Asp 23

蛋白结构（Molecular Characterization） 

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 50.9 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

簇集蛋白（Clusterin，CLU）也称为二聚酸性糖蛋白（DAG蛋白）、睾酮抑制前列腺信息-2（TRPM-2）、硫酸化糖蛋白-2（SGP-2）和补体裂解抑制剂（CLI），是一种分泌型多功能糖蛋白，属于簇集蛋白家族。簇蛋白前体的细胞内裂解会去除信号肽，并产生大小相当的α和β链，这些链以80 kDa N糖基化二硫键连接的异二聚体的形式分泌。DAG蛋白主要在成年睾丸、卵巢、肾上腺、肝脏、心脏和大脑以及胚胎发育过程中的许多上皮组织中表达。聚簇蛋白涉及几个基本的生物学事件，如细胞死亡、肿瘤进展和神经退行性疾病。在不同疾病状态和体外系统中检测到的簇集蛋白mR和蛋白质水平的上调表明，它在膜脂质循环、凋亡细胞死亡以及作为应激诱导的分泌伴侣蛋白等方面发挥作用。