重组人ErbB4/Her4蛋白-ACROBiosystems百普赛斯

Human ErbB4 / Her4 Protein, His Tag

分子别名（Synonym）

HER4,ErbB4,MGC138404,p180erbB4

表达区间及表达系统（Source）

Human ErbB4, His Tag (ER4-H5221) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Pro 651 (Accession # NP_005226.1).

Predicted N-terminus: Gln 26

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.7 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

受体酪氨酸蛋白激酶erbB-4（ErbB4），也称为Her4，是一种单程I型跨膜糖蛋白，是酪氨酸激酶受体erbB家族的成员。ErbB家族成员是表皮生长因子（EGF）家族生长因子的受体。ErbB4在正常骨骼肌、心脏、垂体、脑和几种乳腺癌中表达。ERBB4包含多个富含半胱氨酸的弗林蛋白酶样结构域、一个酪氨酸激酶结构域、磷酸肌醇-3激酶结合位点和一个PDZ结构域结合基序。该蛋白与神经调节蛋白-2和-3、肝素结合EGF样生长因子和β细胞蛋白结合并被其激活。配体结合诱导多种细胞反应，包括有丝分裂和分化。多种蛋白水解事件允许释放细胞质片段和细胞外片段。ErbB4在神经元发育、心脏发育和癌症中起着重要作用。ERBB4已被证明与DLG4、NRG1、STAT5A和YAP1相互作用。该基因的突变与癌症有关。其他单核苷酸多态性和风险单倍型与精神分裂症有关。