重组人CLEC12A蛋白-ACROBiosystems百普赛斯

FITC-Labeled Human CLEC12A / MICL / CLL-1 Protein, His Tag

分子别名（Synonym）

CLEC12A,MICL,CLL-1,CLL1,DCAL2,DCAL-2,CD371

表达区间及表达系统（Source）

FITC-Labeled Human CLEC12A, His Tag (CLA-HF247) is expressed from human 293 cells (HEK293). It contains AA His 65 - Ala 265 (Accession # Q5QGZ9-2). It is the FITC labeled form of Human CLEC12A, His Tag (CLA-H5245).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 25.6 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度（FITC:Protein Ratio)

The FITC to protein molar ratio is 2-4.5.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

CLEC12A（C型凝集素结构域家族12成员A）也称为CLL1、DCAL2、MICL。Clec12a是尿酸晶体的抑制性受体，在细胞死亡时调节炎症。调节信号级联并介导靶MAP激酶酪氨酸磷酸化的细胞表面受体。近年来，骨髓增生异常综合征（MDS）中出现了不同疾病增殖干细胞的证据。然而，CLEC12A在MDS中的作用仍有待阐明。此外，CLEC12A已被提出作为AML中白血病干细胞的有前景的标志物。