重组人CD4蛋白-ACROBiosystems百普赛斯

Human CD4 / LEU3 Protein, His Tag (MALS verified)

分子别名（Synonym）

CD4,CD4mut,LEU3

表达区间及表达系统（Source）

Human CD4, His Tag (LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782).

Predicted N-terminus: Lys 26

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

T细胞表面糖蛋白CD4也称为T细胞表面抗原T4/Leu-3。CD4包含三个Ig样C2型（免疫球蛋白样）结构域和一个Ig样V型（免疫蛋白样）功能域。CD4是MHC II类抗原/T细胞受体相互作用的辅助蛋白。CD4诱导脂筏聚集。CD4是HIV-1用于进入宿主T细胞的主要受体。HIV感染导致具有CD4受体的T细胞数量逐渐减少。因此，医疗专业人员参考CD4计数来决定何时开始治疗HIV感染患者。