重组人KRAS蛋白-ACROBiosystems百普赛斯

Human KRAS (2-185, G12C) Protein, His Tag (active enzyme)

分子别名（Synonym）

GTPase Kras,K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras,KRAS2,RASK2,C-K-RAS,CFC2,K-RAS2A,K-RAS2B,K-RAS4A,K-RAS4B,KI-RAS,KRAS1,KRAS2,NS,NS3,RASK2,KRAS

表达区间及表达系统（Source）

Human KRAS (2-185, G12C) Protein, His Tag (KRS-H51H3) is expressed from E. coli cells. It contains AA Thr 2 - Cys 185 (Accession # P01116-2 (G12C)).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 23.0 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

KRAS（Kirsten大鼠肉瘤2病毒癌基因同源物）基因是一种原癌基因，编码一种名为KRAS的小GTP酶转导蛋白。KRAS也被称为Ki-Ras、c-K-Ras和c-Ki-ras。Ras蛋白结合GDP/GTP并具有内在的GTP酶活性。在调节细胞增殖中发挥重要作用，通过以ZNF304依赖的方式诱导癌症（CRC）细胞中肿瘤抑制基因（TSG）的转录沉默来促进致癌事件。RAS是人类癌症中最常见的突变癌基因之一，但RAS基因突变的频率和分布并不均匀。具体来说，甘氨酸12（G12）的突变通过干扰GAP结合和GAP刺激的GTP水解来引起RAS激活。参考文献表明，该途径可能成为潜在的治疗靶点。