重组人Sortilin/SORT1蛋白-ACROBiosystems百普赛斯

Biotinylated Human Sortilin / SORT1 Protein, His,Avitag™ (MALS & SPR verified)

分子别名（Synonym）

Sortilin,SORT1,100 kDa NT receptor,Glycoprotein 95,Gp95,Gp95LDLCQ6,Neurotensin receptor 3,NT3NTR3,Ntr3,sortilin 1

表达区间及表达系统（Source）

Biotinylated Human Sortilin Protein, His,Avitag (SON-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Asn 755 (Accession # Q99523-1).

Predicted N-terminus: Ser 78

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 79.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Sortilin是高尔基体隔室中的分选受体，也是细胞表面的清除受体。通过独立于甘露糖-6-磷酸受体（M6PR）的途径将蛋白质从高尔基体运输到溶酶体所需。溶酶体蛋白特异性结合高尔基体中的受体，产生的受体-配体复合物被转运到酸性前溶酶体室，在那里低pH值介导复合物的解离。然后，受体通过与逆转录酶的结合被再循环回高尔基体进行另一轮运输。也是蛋白质从高尔基体运输到内体所必需的。通过介导促凋亡前体BDNF（proBDNF）和NGFB（proNGFB）的内吞作用促进神经元凋亡。也充当神经降压素的受体。可能通过清除细胞外LPL促进成骨分化过程中细胞外基质的矿化。脂肪细胞可能需要形成含有葡萄糖转运蛋白SLC2A4/GLUT4的特殊储存囊泡（GLUT4储存囊泡或GSV）。这些囊泡提供了稳定的SLC2A4池，并提高了对胰岛素的反应性。也可能介导从内质网到高尔基体的运输。