重组人TNFR1蛋白-ACROBiosystems百普赛斯

Human TNFR1 / CD120a / TNFRSF1A Protein, His Tag

分子别名（Synonym）

TNFR1,CD120a,TNFRSF1A,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR55,TNFR60,p55,p60

表达区间及表达系统（Source）

Human TNFR1, His Tag (TN1-H5222) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Thr 211 (Accession # NP_001056).

Predicted N-terminus: Ile 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 28-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

TNFR1也称为TNFRSF1A，属于TNF受体超家族。TNFR1包含一个死亡结构域和四个TNFR-Cys重复序列。TNF-R1是TNFSF2/TNFα和同源三聚体TNFSF1/淋巴毒素-α的受体。衔接分子FADD将胱天蛋白酶-8募集到活化的受体上。由此产生的死亡诱导信号复合物（DISC）进行胱天蛋白酶-8蛋白水解激活，启动随后介导凋亡的胱天蛋白酶（天冬氨酸特异性半胱氨酸蛋白酶）级联反应。TNFR1有助于诱导非细胞杀伤性TNF效应，包括抗病毒状态和酸性鞘磷脂酶的激活。TNFRSF1A的缺陷是家族性冬眠热（FHF）的原因。