重组人STAT3蛋白-ACROBiosystems百普赛斯

Human STAT3 Protein, His Tag (MALS & SPR verified)

分子别名（Synonym）

STAT3, signal transducer and activator of transcription 3

表达区间及表达系统（Source）

Human STAT3, His Tag (ST3-H5149) is expressed from E. coli cells. It contains AA Gly 127 - Arg 688 (Accession # P40763-1).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 66.4 kDa. The protein migrates as 66-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

信号转导子和转录激活子3（STAT3）调节对基本细胞过程（包括存活、增殖、自我更新、血管生成和免疫反应）重要的基因的表达。作为早期肿瘤诊断标志物，组成型STAT3激活被认为是模型系统中引起恶性转化和肿瘤发展的充分因素。癌症细胞中经典的IL6/STAT3信号通路。IL-6与膜结合的IL-6受体α（IL-6R）和IL-6受体β（也称为gp130）结合。IL-6/IL-6R/gp130复合物激活JAKs的磷酸化，随后是STAT3的磷酸化和激活。然后，磷酸化的STAT3形成同二聚体并易位到细胞核中，与靶基因的启动子区域结合并激活靶基因转录。