重组人APOE/Apolipoprotein E蛋白-ACROBiosystems百普赛斯

Human Apolipoprotein E / APOE3 Protein, His Tag

分子别名（Synonym）

Apolipoprotein E,APOE,Apo-E

表达区间及表达系统（Source）

Human APOE3, His Tag (APE-H5246) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1).

Predicted N-terminus: Lys 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

无菌（Sterility）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

APOE是一种载脂蛋白，一种与脂质颗粒相关的蛋白质，主要通过血浆和间质液在脂蛋白介导的器官间脂质运输中发挥作用。载脂蛋白E是血浆脂蛋白的核心成分，参与其产生、转化和清除。载脂蛋白是两亲性分子，与脂蛋白颗粒核心的脂质和血浆的水环境相互作用。因此，APOE与乳糜微粒、乳糜微粒残留、极低密度脂蛋白（VLDL）和中密度脂蛋白。它还与多种细胞受体结合，包括LDL受体/LDLR、LDL受体相关蛋白LRP1、LRP2和LRP8以及极低密度脂蛋白受体/VDLR，这些受体介导含APOE的脂蛋白颗粒的细胞摄取。