重组人Mucin-17/MUC17蛋白-ACROBiosystems百普赛斯

Human MUC17 Protein, His Tag

分子别名（Synonym）

Mucin-17,MUC-17,MUC17,MUC-3,MUC3

表达区间及表达系统（Source）

Human MUC-17, His Tag (MU7-H52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 4131 - Leu 4390 (Accession # Q685J3-1).

Predicted N-terminus: Arg 4131 & 4244 Ser

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein was cleaved within the SEA domain between 4243 Gly and 4244 Ser, and was cleaved into N and C-terminal fragment with calculated MW of 12.6 kDa and 18.6 kDa respectively. The protein migrates as 20 kDa and 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

膜粘蛋白在上皮细胞中具有多种功能，包括细胞保护、转移过程中的外渗、维持管腔结构和信号转导。MUC17包含一个延伸的、重复的细胞外糖基化结构域和一个羧基末端，羧基末端有两个EGF样结构域、一个SEA模块结构域、另一个跨膜结构域和具有潜在丝氨酸和酪氨酸磷酸化位点的细胞质结构域。通过其C末端与PDZK1相互作用，这种相互作用对于正确定位非常重要。可能在维持粘膜表面的稳态方面发挥作用。