重组人EpCAM/TROP1蛋白-ACROBiosystems百普赛斯

Human EpCAM / TROP1 Protein, His Tag

分子别名（Synonym）

EPCAM,TACSTD1,TROP1,CD326,DIAR5,EGP2,EGP314,EGP40,ESA,GA733-2,HNPCC8,HNPCC-8,KS1,4,KSA,M4S1,MIC18,MK1

表达区间及表达系统（Source）

Human EpCAM, His Tag (EPM-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).

Predicted N-terminus: Gln 24

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.2 kDa. The protein migrates as 31-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

EpCAM也被称为CO171A、EGP、EGP40、GA7332、KSA、M4S、MIC18、MK1、TROP1、hEGP2，是一种泛上皮分化抗原，几乎在所有癌症上都以17-1A（mAb）抗原的形式表达。其宪法功能正在被阐明。它与钙粘蛋白-Catenin通路错综复杂地联系在一起，因此是负责细胞内信号传导和极性的基本WNT通路。上皮细胞粘附分子（Ep-CAM）已知在大多数上皮恶性肿瘤中表达，并被报道为肿瘤标志物或分子靶向治疗的候选者。

据报道，Ep-CAM与N-钙粘蛋白的交叉信号传导涉及Pi3K，导致上皮细胞中钙粘蛋白粘附复合物的消除。上皮细胞粘附分子（Ep-CAM）作为癌症的一个预后因子，最近受到越来越多的关注。