重组人GUCY2C蛋白-ACROBiosystems百普赛斯

Human GUCY2C / Guanylyl cyclase C Protein, His Tag (MALS verified)

分子别名（Synonym）

GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C

表达区间及表达系统（Source）

Human GUCY2C, His Tag (GUC-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).

Predicted N-terminus: Ser 24

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

GUCY2C（Guanylyl Cyclase C），也称为热稳定肠毒素受体，是鸟苷酸环合酶（gc）家族的I型跨膜蛋白，通过产生cGMP发出信号。鸟苷酸环化酶C（GUCY2C）及其激素鸟苷酸和尿鸟苷酸最近成为一个旁分泌轴，保护肠粘膜完整性免受突变、化学和炎症损伤。GUCY2C小鼠CAR-T细胞识别并杀死内源性表达GUCY2C的人结直肠癌癌症细胞。因此，我们已经确定了一种人类GUCY2C-特异性CAR-T细胞治疗方法，该方法可能被开发用于治疗GUCY2C外转移性癌症。