重组人IL2RA蛋白-ACROBiosystems百普赛斯

Human IL-2 R alpha / CD25 Protein, His Tag

分子别名（Synonym）

IL2RA,CD25,p55,IL2-RA,IL-2-RA

表达区间及表达系统（Source）

Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).

Predicted N-terminus: Glu 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白细胞介素-2受体亚基α（IL2RA）也称为IL-2R亚基α、IL-2-RA、IL2-RA、TAC抗原、p55、CD抗原CD25，是一种I型跨膜糖蛋白。IL2RA在活化的T细胞和调节性T细胞上表达，并且能够自身以低亲和力结合IL2。然而，当IL2RA与IL2受体β链和γ链非共价结合，随后启动MAPK或JAK/STAT等细胞内信号通路时，会产生配体诱导的高亲和力异三聚体受体复合物。在树突状细胞（DC）上，CD25以前被认为是一种激活标志物，而小鼠和人类DC都可以表达CD25，但它们不表达IL-2受体的β链，这是执行IL-2信号传导所必需的。