重组人RANKL/TNFSF11蛋白-ACROBiosystems百普赛斯

Human TNFSF11 / RANKL / CD254 Protein, His Tag, active trimer (MALS verified)

分子别名（Synonym）

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统（Source）

Human TNFSF11 Protein, His Tag (RAL-H5240) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.4 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

核因子κB配体受体激活剂（RANKL），也称为肿瘤坏死因子配体超家族成员11（TNFSF11）、TNF相关活化诱导细胞因子（TRANCE）、骨保护素配体（OPGL）和破骨细胞分化因子（ODF），被称为II型膜蛋白，是肿瘤坏死因子（TNF）超家族的成员。RANKL通过其刺激破骨细胞形成和活性的能力，是骨吸收和整体骨密度的关键介质。一些研究结果还表明，一些癌症细胞，特别是癌症细胞，可以激活骨重塑的增加，并最终增加总的骨生成。骨重塑和骨生成的增加增加了骨转移的整体生长。骨重塑的总体控制是通过RANKL与其受体或其诱饵受体RANK和OPG的结合来调节的。