重组人补体C5（Complement C5）蛋白-ACROBiosystems百普赛斯

Human Complement C5 Protein, His Tag (MALS verified)

分子别名（Synonym）

Complement C5,C5,CPAMD4

表达区间及表达系统（Source）

Human Complement C5, His Tag (CO5-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).

Predicted N-terminus: Gln 19 (β chain) & Thr 678 (α chain)

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 66 kDa, 110 kDa and >180kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and >180 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

C5过敏毒素来源于补体C5的蛋白水解降解，是局部炎症过程的介质。C5前体首先通过去除4个碱性残基进行加工，形成由二硫键连接的两条链，β和α。C5转化酶通过切割α链激活C5，释放C5a过敏毒素并产生C5b（β链+α'链）。C5转化酶激活C5启动晚期补体成分C5-C9自发组装成膜攻击复合物。C5b具有C6的瞬时结合位点。C5b-C6络合物是裂解络合物组装的基础。C5a过敏毒素与C5AR1和蜱补体抑制剂相互作用。C5a也是一种强效的趋化因子，可以刺激多形核白细胞的运动，并引导它们向炎症部位迁移。